Mystique - microorganiam/bacteria tests

Sludge Reduction Study on Wastewater Pond at a Municipal Treatment Facility with Industry

Background:
The project requirement was treatment of creamery and septage waste discharged to wasting ponds. Lowering solids accumulated on the bottom of the primary pond and odor control were the objectives. The primary wasting pond measures 1200' length x 200' width x 8' depth.
Approach and Application of the Technology:
Before Bioaugmentation was started (May 15 1996), the pond was poked with a "Sludge Judge" to determine the amount of sludge that had built up. On the following week a four month program of adding microorganisms "Mystique" was started. There was ane aerator at the north end of the pond that operated during the test.
Results:
dddAfter a four month program of adding Mystique, the amount of sludge was reduced by an average of 9.12 inches, with the largest amount of sludge (41") digested near the inlet of the pond which is where the Mystique was added. During the second month of the test a reduction in the amount of floating material and reduction of odor was noted. By the end of the third month a purple cast and larger area of clear water was apparent. At the end of the four month test, the pond had gone under a dramatic reduction of sludge and the septic smell was reduced to nill.
Summary:
Mystique is capable of reducing solids, thus, eliminating dredging costs. Mystique is capable of substantial odor reduction.

May 16, 1996 NORTH
1" 1" 1" inlet
1" 6" 3"
WEST 12" 18" 12" EAST
42" 42" 36"
60" 60" 60"
SOUTH

Sept. 18, 1996 NORTH
42" 30" 33" inlet
9" 9" 9"
WEST 21" 21" 21" EAST
39" 39" 33"
51" 51" 51"
SOUTH



















Biotreatability of Dairy Whey Waste Combined with Winery Waste

Background:
The project requirement was to biologically treat a mixture of dairy whey and winery waste before discharge to a municipal sewer system. The waste had an acidic pH (4.97) and a very strong noxious odor.
Approach and Application of the Technology:
The pH of the waste was first adjusted to 7.5 using sodium hydroxide. The waste was then treated at 28 degrees Celsius with aeration. Two tests were conducted: Indigenous Microbial Treatment
Addition of supplemental nutrients and abiological surfactant, and
Bioaugmented Treatment
Addition of supplemental nutrients, a biological surfactant and Mystique
Results:
Biochemical Oxygen Demand (BOD) was measured in the original waste and following 14 days of treatment. The Indigenous Microbial Treatment showed a 22% reduction in BOD, while the Bioaugmented Treatment using Mystique showed a 71% reduction in BOD. Additionally odor was significantly reduced within 24 hours in the Bioaugmented Treatment.



Pretreatment of Grease Trap and Septage Waste

Background:
The project requirement was pretreatment of grease trap and septage waste for discharge into a municipal wastewater treatment facility. Grease trap waste, in particular, creates many problems for treatment facilities. Most municipal facilities are not equipped nor designed to handle this type of waste. Previously, grease trap waste has been deposited in regional landfill leachate ponds. This type of facility has little effect on the fats, oils and grease (FOG) contained in this waste and eventually the waste escapes the pond polluting receiving streams and rivers. A system of pretreatment was needed to reduce the FOG and Solids content of the waste.
Approach and Application of the Technology:
The approach was to utilize a unique, patented, venturi aeration device coupled with bioaugmentation regime using proprientary microorganisms. These microorganisms have been selectively adapted for degradation of fats, oils and grease. In the pretreatment design, grease trap waste is diluted with septage waste and bioaugmented with Mystique. Mixing and aeration are achieved using the appropriately sized venturi aeration device and pH and foam control are maintained by automatic sensing and metering equipment.
Results:
The pretreatment design performed well in the actual field operation. A 4000 gallon pilot study performed in cooperation with a large Southeastern Utilities Authority. In this project Total Suspended Solids were reduced by 58%, Biochemical Oxygen Demand was reduced by 80%, Chemical Oxygen Demand was reduced by 63% and Fats, Oils and Grease were reduced by 96%. This successful pretreatment allows


Biotreatability Study on Wastewater from a Candy Manufacturing Facility

Background:
The project requirement was treatment of wastewater from an international candy manufacturer.
Two types of water were treated:
1. Normal Wasterwater, and
2. Sugar-Water Wastewater
Approach and Application of the Technology:
Triplicate samples of both raw wastewater and sugar wastewater were incubated for 5 days at 25 degrees Celsius with aeration. Each sample was bioaugmented with Mystique. Initial samples were taken and BOD and Solids were determined. BOD and Solids were also determined following the 5 day treatment period.
Results:
In the five day period BOD was reduced by 83% in both raw wastewater and in sugar wastewater. Solids were reduced by 92% in the raw wastewater and 98% in the sugar wastewater.
Summary:
Mystique is capable of dramatic BOD reductions in high(150,000) wastes, including high osmotic pressure sugar water that is often lethal to other microoganisms.
Mystique is capable of dramatic Solids reduction in high osmotic pressure sugar water.




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